An intein-split transactivator for intersectional neural imaging and optogenetic manipulation.
Chen HS, Zhang XL, Yang RR, Wang GL, Zhu XY, Xu YF, Wang DY, Zhang N, Qiu S, Zhan LJ, Shen ZM, Xu XH, Long G, Xu C
Nat Commun. 2022 Jun 23;13(1):3605. doi: 10.1038/s41467-022-31255-x.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172119 | pRabies-EGFP-rtTAn | Expression of EGFP and rtTA-InteinN in Rabies virus infected cells |
| 172120 | pRabies-EBFP-tTAn | Expression of EBFP and tTA-InteinN in Rabies virus infected cells |
| 172121 | pAAV-pEF1a-rtTAc | Constitutive expression of InteinC-rtTAC |
| 172122 | pAAV-pEF1a-EBFP-tTAn | Constitutive expression of EBFP and rtTA-InteinN |
| 172123 | pAAV-pEF1a-tTAc | Constitutive expression of InteinC-tTAC |
| 172124 | pAAV-pCaMKII-EBFP-tTAn | Expression of EBFP and tTA-InteinN in CaMKII positive cells |
| 172125 | pAAV-pCaMKII-tTAc | Expression of InteinC-tTAC in CaMKII positive cells |
| 172126 | pAAV-pEF1a-DIO-tTAc | Expression of InteinC-tTAC in Cre recombinase positive cells |
| 172127 | pAAV-EF1a-FRT-EBFP-tTAn | Expression of EBFP and tTA-InteinN in Flp recombinase positive cells |