A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation.
Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S
Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172100 | pCMV-EGFP-eDHFR(69K6)-MCS | Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6) |
| 172101 | pCMV-MCS-eDHFR(69K6)-EGFP | Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP |
| 172102 | pPBpuro-mNG-eDHFR(69K6)-Tiam1 | Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6) |
| 172103 | pPBpuro-mNG-eDHFR(69K6)-p85iSH2 | Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6) |
| 172104 | pCSIIpuro-mNG-eDHFR(69K6)-RasGEF | Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6) |
| 172105 | pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s | Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6) |
| 172106 | pCAGGS-mNG-eDHFR(69K6)-G-alpha-q | Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6) |
| 172107 | pPBpuro-cRaf-mNG-eDHFR(69K6) | Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6) |
| 178849 | pPBpuro-EGFP-eDHFR(69K6)-cRaf | Mammalian expression of cRaf fused to EGFP-eDHFR(69K6) |
| 178850 | pPBpuro-EGFP-eDHFR(69K6) | Mammalian expression of EGFP-eDHFR(69K6) |
| 178851 | pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A) | Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6) |
| 178852 | pCSIIpuro-miRFP670-eDHFR(69K6) | Mammalian expression of miRFP670-eDHFR(69K6) |
| 178853 | pCSIIpuro-mNG-eDHFR(69K6) | Mammalian expression of mNeonGreen-eDHFR(69K6) |
| 178854 | pCAGGS-chGrb2/eDHFR(69K6)-iRFP713 | Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713 |
| 178855 | pCAGGS-eDHFR(69K6)-iRFP713 | Mammalian expression of eDHFR(69K6)-iRFP713 |
| 178856 | pCAGGS-PKCdelta-EGFP | Mammalian expression of PKCδ fused to EGFP |
| 178857 | pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP | Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP |
| 178858 | pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670 | Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670 |
| 178859 | pCMV-miRFP670-eDHFR(69K6) | Mammalian expression of miRFP670-eDHFR(69K6) |
| 178860 | pCAGGS-EGFP-SOS1 | Mammalian expression of SOS1 fused to EGFP |