Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila.
Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, Xie J, Kamiyama D
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 10.1073/pnas.2024690118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172061 | pACUH-sfcherry2(1-10) | UAS vector to express sfcherry2(1-10) in Drosophila |
| 172062 | pACUH-GFP1-10 sec | UAS vector to express GFP1-10 in the ER lumen |
| 172063 | pACUH-GFP1-10 mitochondrial matrix | UAS vector to express GFP1-10 in the mitochondrial matrix |
| 172064 | pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7) | Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron |
| 172065 | pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7) | Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron |
| 172066 | pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7) | Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron |
| 172067 | pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7 | Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron |
| 172068 | pBS-KS-attB1-2-PT-SA-SD-1-GFP11 | Protein trap plasmid for inserting GFP11 into a phase 1 coding intron |