A photo-switchable yeast isocitrate dehydrogenase to control metabolic flux through the citric acid cycle
Chen H, Mulder L, Wijma HJ, Wabeke R, Vila Cha Losa JP, Rovetta M, de Leeuw TC, Milias-Argeitis A, Heinemann M
bioRxiv
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166101 | pROS13_IDH1 #4 | This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination. |
| 166102 | pROS13_IDH2 #7 | This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination. |