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Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor.

Wang K, Wang H, Li C, Yin Z, Xiao R, Li Q, Xiang Y, Wang W, Huang J, Chen L, Fang P, Liang K
Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe3516. doi: 10.1126/sciadv.abe3516. Print 2021 Feb. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171869pGEX-GST-His6-1_HBDExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells
171870pGEX-GST-His6-2_HBDExpresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells
171871pA-His6-Tn5Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Antibodies from Article