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CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.

Barber KW, Shrock E, Elledge SJ
Mol Cell. 2021 Aug 4. pii: S1097-2765(21)00596-7. doi: 10.1016/j.molcel.2021.07.027. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171798pdCas9+sgRNA expression vector precursorPICASSO: Library expression vector precursor for paired dCas9+sgRNA expression
171799Expression scaffold for dCas9+sgRNAPICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

Antibodies from Article