CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
Barber KW, Shrock E, Elledge SJ
Mol Cell. 2021 Aug 4. pii: S1097-2765(21)00596-7. doi: 10.1016/j.molcel.2021.07.027.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171798 | pdCas9+sgRNA expression vector precursor | PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression |
| 171799 | Expression scaffold for dCas9+sgRNA | PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA |