Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines.
Tromp A, Wang H, Hall TE, Mowry B, Giacomotto J
Front Physiol. 2023 Aug 2;14:1221310. doi: 10.3389/fphys.2023.1221310. eCollection 2023.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171792 | 469_pME_iCre | pME-Vector with codon improved iCre sequence. Tol2-kit compatible. |
| 171793 | 141_pT3TS_iCre | Plasmid for optimised iCre RNA synthesis using T3 promoter. |
| 171794 | 1455_pDEST_miniTol2_R4-R2_MCS | pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion. |
| 171795 | 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP | pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes) |
| 171796 | 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry | pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes) |
| 171797 | 114_UBI_iCre_crystGFP | Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-) |