Fluorescence Lifetime Imaging of pH along the Secretory Pathway.
Linders PTA, Ioannidis M, Ter Beest M, van den Bogaart G
ACS Chem Biol. 2022 Jan 21;17(1):240-251. doi: 10.1021/acschembio.1c00907. Epub 2022 Jan 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171716 | pRpHLuorin2-N1 | Ratiometric pHLuorin2 backbone (C terminal fusion) |
| 171717 | ER-RpHLuorin2 | Luminal ER expression of ratiometric pHLuorin2 |
| 171718 | MGAT2-RpHLuorin2 | Cis-/medial-Golgi expression of ratiometric pHLuorin2 |
| 171719 | GalT-RpHLuorin2 | Trans-Golgi expression of ratiometric pHLuorin2 |
| 171720 | LAMP1-RpHLuorin2 | Lysosomal expression of ratiometric pHLuorin2 |
| 171721 | GPI-RpHLuorin2 | GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular) |
| 171722 | Str-KDEL_TNF-SBP-RpHLuorin2 | Synchronize trafficking of TNFa-RpHLuorin2 |
| 180227 | RpHLuorin2 | for bacterial expression of recombinant ratiometric pHluorin2 |