Protein expression and purification of G-protein coupled receptor kinase 6 (GRK6), toward structure-based drug design and discovery for multiple myeloma.
Olson TL, Zhang S, Labban D, Kaschner E, Aceves M, Iyer S, Meza D, Zook JD, Chun E, Craciunescu FM, Liu W, Shi CX, Stewart AK, Hansen DT, Meurice N, Fromme P
Protein Expr Purif. 2021 May 7:105890. doi: 10.1016/j.pep.2021.105890.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157736 | pFastBac1-His10FLAG | empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites |
| 159427 | pFastBac1-His10TEV | empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites |