Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator.

MacDonald IC, Seamons TR, Emmons JC, Javdan SB, Deans TL
Nat Commun. 2021 Jul 5;12(1):4109. doi: 10.1038/s41467-021-24434-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
171648	T7-LacO-GFP_ColE1	Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.
171649	QUAS-0-T7-GFP_ColE1	QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171650	T7-0-QUAS-GFP_ColE1	QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171651	T7-LacO-QF_p15A	Expresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.
171652	QUAS-0-T7-CcdB_ColE1	QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171653	QUAS-5-T7-GFP_ColE1	QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171654	QUAS-10-T7-GFP_ColE1	QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171655	QUAS-15-T7-GFP_ColE1	QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171656	T7-5-QUAS-GFP_ColE1	QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171657	T7-10-QUAS-GFP_ColE1	QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171658	T7-15-QUAS-GFP_ColE1	QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.
171659	T7-TetO-QF_T7-LacO-TetR_p15A	Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.
171660	QUAS-5-T7-TetO-GFP_ColE1	QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.
171661	T7-LacO-QF-T7-TetO-TetR_p15A	Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.
171662	QUAS-0-T7-TetO-GFP_ColE1	QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.
171663	QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1	QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
171664	T7-LacO-TetR_p15A	Expression of TetR in BL21(DE3) E. coli. Contains p15A origin of replication.
173193	T7-TetO-GFP_ColE1	Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.