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Tagging active neurons by soma-targeted Cal-Light.

Hyun JH, Nagahama K, Namkung H, Mignocchi N, Roh SE, Hannan P, Krussel S, Kwak C, McElroy A, Liu B, Cui M, Lee S, Lee D, Huganir RL, Worley PF, Sawa A, Kwon HB
Nat Commun. 2022 Dec 12;13(1):7692. doi: 10.1038/s41467-022-35406-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171615pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTASoma-targeted Cal-Light with KA2
171616pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTASoma-targeted Cal-Light with Kv2.1
171617pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTAAAV Soma-targeted Cal-Light with KA2
171618pAAV-pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTAAAV Soma-targeted Cal-Light with Kv2.1
171619pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PAAAV floxed Soma-targeted Cal-Light with KA2
171620pAAV-M13-TevC-IRES-SP6-CreM13-TevC with Cre
171621pAAV-TRE-NpHR-EYFPTRE-NpHR-EYFP reporter
171622pAAV-TRE-ChR2-YFPTRE-ChR2-YFP reporter

Antibodies from Article