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Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1.
Mickolajczyk KJ, Olinares PDB, Niu Y, Chen N, Warrington SE, Sasaki Y, Walz T, Chait BT, Kapoor TM
Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. doi: 10.1073/pnas.2002792117. Epub 2020 Jul 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171602Mdn1-FLExpresses full length S. pombe Mdn1 in insect cells.
171603Mdn1-deltaCExpresses S. pombe Mdn1 a.a. 1-3911 in insect cells.
171604SNAP-MIDASExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.
171605deltaLoop-MIDASExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

Antibodies from Article