Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells.

Mendez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker J, Robb GB, Holden K, Sanjana NE
Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
171585	pLenti-TRS-Leader-SARS-CoV-2-EGFP	Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter
171586	pET28-His-MBP-NLS-RfxCas13d-NLS-HA	Bacterial expression of NLS-RfxCas13d-NLS-HA
171587	pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His	Bacterial expression of NLS-RfxCas13d-NLS 6His