Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells.
Mendez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker J, Robb GB, Holden K, Sanjana NE
Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171585 | pLenti-TRS-Leader-SARS-CoV-2-EGFP | Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter |
| 171586 | pET28-His-MBP-NLS-RfxCas13d-NLS-HA | Bacterial expression of NLS-RfxCas13d-NLS-HA |
| 171587 | pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His | Bacterial expression of NLS-RfxCas13d-NLS 6His |