Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies.
Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh HL, Perrimon N
Elife. 2022 Jan 25;11. pii: 74326. doi: 10.7554/eLife.74326.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171564 | pMT-HGP-v3-Nb127D01-hIgG | Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells |
| 171565 | pMT-HGP-v3-NbVHH05-hIgG | Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells |
| 171566 | pET-26b-Nb127D01-HA-His | Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His) |
| 171567 | pET-26b-NbVHH05-HA-His | Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His) |
| 171568 | pET-26b-Nb127D01-ALFA-His | Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His) |
| 171569 | pET-26b-NbVHH05-ALFA-His | Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His) |
| 171570 | pAW-NbVHH05-GFP | Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells |
| 171571 | pAW-Nb127D01-GFP | Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells |
| 171572 | pAW-NbVHH05-mCherry | Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells |
| 171573 | pAW-Nb127D01-mCherry | Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells |
| 171574 | pAW-BiP-NbVHH05-mCherry-KDEL | Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane |
| 171575 | pAW-BiP-Nb127D01-mCherry-KDEL | Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane |
| 171576 | pAW-CD8-NbVHH05-GFP | Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane |
| 171577 | pAW-CD8-Nb127D01-GFP | Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane |
| 171578 | pScarlessHD-C-3x127D01-DsRed | For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini. |
| 171579 | pScarlessHD-N-3x127D01-DsRed | For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini. |
| 171580 | pScarlessHD-C-3xVHH05-DsRed | For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini. |
| 171581 | pScarlessHD-N-3xVHH05-DsRed | For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini. |