Extending the L1 region in canonical double-stranded RNA-binding domains impairs their functions.
Zhang X, Li P, Zhong H, Yang F, Liu F, Yedid G, Zeng Y
Acta Biochim Biophys Sin (Shanghai). 2021 Mar 26;53(4):463-471. doi: 10.1093/abbs/gmab014.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171541 | pCMV-HA-DGCR8(+6) | Encodes mutant human DGCR8 protein |
| 171542 | pGEX-4T-1-DGCR8-2xdsRBD(+6) | Encodes a DGCR8 fragment extending from E502 to the C-terminus |
| 171543 | pGEX-4T-1-Dicer | Encodes a Dicer fragment extending from from R1841 to the C-terminus |
| 171544 | pGEX-4T-1-Dicer-dsRBD (+6) | Encodes a Dicer fragment extending from from R1841 to the C-terminus |
| 171545 | pGEX-4T-1-Staufen | Encoding part of the Drosophila Staufen protein from M571 to P656 |
| 171546 | pGEX-4T-1-Staufen-dsRBD(+6) | Encoding part of the Drosophila Staufen protein from M571 to P656 |
| 171547 | pGEX-4T-1-Xlrbpa | Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202 |
| 171548 | pGEX-4T-1-Xlrbpa-dsRBD(+6) | Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202 |
| 171549 | pGEX-4T-1-Rnt1-dsRBD | Encoding part of the budding yeast Rnt1 protein from L366 to S453 |
| 171550 | pGEX-4T-1-Rnt1-dsRBD(+6) | Encoding part of the budding yeast Rnt1 protein from L366 to S453 |
| 171551 | pGEX-4T-1-Rnt1-dsRBD(+3A) | Encoding part of the budding yeast Rnt1 protein from L366 to S453 |
| 171552 | pGEX-4T-1-Rnt1-dsRBD(+6A) | Encoding part of the budding yeast Rnt1 protein from L366 to S453 |