Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy.
Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, Hurley JH
Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 10.1126/sciadv.abg4922. Print 2021 Apr.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171410 | GST-TEVcs-FIP200-MBP | For the purifcation of human ULK1 complex |
| 171411 | GST-TEVcs- FIP200 delta641-779-MBP | For the purifcation of human ULK1 truncated complex |
| 171412 | ATG13 | For the purifcation of human ULK1 complex |
| 171413 | GFP-ATG13 | For the purifcation of human ULK1 complex |
| 171414 | GST-TEVcs-ATG101 | For the purifcation of human ULK1 complex |
| 171415 | GST-TEVcs-GFP-ATG101 | For the purifcation of GFP-ULK1 complex |
| 171416 | MBP-TSF-TEVcs-ULK1 | For the purifcation of human ULK1 complex |
| 171417 | MBP-TSF-TEVcs-ULK1K46I | For the purifcation of human ULK1 kinase dead complex |
| 171418 | VPS15 | For the purifcation of human PI3KC3 complex |
| 171419 | WIPI2d-TEVcs-TSF | For the purifcation of WIPI2d protein |
| 171420 | GFP-WIPI2d-TEVcs-TSF | For the purifcation of GFP- WIPI2d protein |
| 171421 | GST-Ub4 | For the purifcation of tetra linear ubiquitin |
| 171422 | GST-NDP52 | For the purifcation of NDP52 protein |
| 171423 | GST-TAX1BP1 | For the purifcation of TAX1BP1 protein |
| 171424 | GST-OPTN | For the purifcation of OPTN protein |
| 171425 | GST-OPTNS177DS473D | For the purifcation of phosphorylation mimic protein |
| 171426 | GST-GFP-ATG14 | For the purifcation of GFP- PI3KC3 complex |