Skip to main content
Addgene

ATP13A2 deficiency disrupts lysosomal polyamine export.

van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, Eggermont J, Vangheluwe P
Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. Epub 2020 Jan 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171377pYeDP60-hATP13A2-WTA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
171378pYeDP60-hATP13A2-E343AA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
171485pLenti HsATP13A2 WTtransfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT
171771miR3 Mm ATP13A2transfer plasmid for lentiviral vector production with miR for Mm ATP13A2
171820pLenti HsATP13A2 D508Ntransfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N
171821pLenti HsATP13A2 D962Ntransfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N
171822miR5 Mm ATP13A2transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Antibodies from Article