Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli.
Okano K, Sato Y, Hizume T, Honda K
J Biosci Bioeng. 2021 Aug;132(2):120-124. doi: 10.1016/j.jbiosc.2021.04.009. Epub 2021 May 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171182 | pCas12f1 | Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1 |
| 171183 | pTarget12f1 | Constitutive expression of sgRNA without donor DNA |