Interactions between AMOT PPxY Motifs and NEDD4L WW Domains Function in HIV-1 Release.

Rheinemann L, Thompson T, Mercenne G, Paine EL, Peterson FC, Volkman BF, Alam SL, Alian A, Sundquist WI
J Biol Chem. 2021 Jul 17:100975. doi: 10.1016/j.jbc.2021.100975. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
171137	pCI-Flag-NEDD4L∆WW1,2,4	expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
171130	pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)	expresses AMOT(E104K/E105K) in mammalian cells
166715	pcDNA3HA-AMOT p130∆PPxY1(A112K)	expresses AMOT(A112K) in mammalian cells
166714	pcDNA3HA-AMOT p130∆PPxY1(L106P)	expresses AMOT(L106P) in mammalian cells
166713	pcDNA3HA-AMOT p130∆PPxY3	expresses AMOT with inactivating mutation in third PPxY motif in mammalian cells
166712	pcDNA3HA-AMOT p130∆PPxY2	expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells
166711	pcDNA3HA-AMOT p130∆PPxY1	expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells
166710	pCI-Flag-NEDD4L∆WW4	expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells
166709	pCI-Flag-NEDD4L∆WW3	expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells
166708	pCI-Flag-NEDD4L∆WW2	expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells