Interactions between AMOT PPxY Motifs and NEDD4L WW Domains Function in HIV-1 Release.
Rheinemann L, Thompson T, Mercenne G, Paine EL, Peterson FC, Volkman BF, Alam SL, Alian A, Sundquist WI
J Biol Chem. 2021 Jul 17:100975. doi: 10.1016/j.jbc.2021.100975.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166701 | pGEX-PP-NEDD4L-WW1(193-226) | expresses NEDD4L WW1 in bacterial cells |
| 166702 | pGEX-PP-NEDD4L-WW2(385-418) | expresses NEDD4L WW2 in bacterial cells |
| 166703 | pGEX-PP-NEDD4L-WW3(497-530) | expresses NEDD4L WW3 in bacterial cells |
| 166704 | pGEX-PP-NEDD4L-WW4(548-581) | expresses NEDD4L WW4 in bacterial cells |
| 166705 | pCA528-PPxY1-GS-WW3e | expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells |
| 166706 | pCA528-AMOT(1-300)-sortase | expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells |
| 166707 | pCI-Flag-NEDD4L∆WW1 | expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells |
| 166708 | pCI-Flag-NEDD4L∆WW2 | expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells |
| 166709 | pCI-Flag-NEDD4L∆WW3 | expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells |
| 166710 | pCI-Flag-NEDD4L∆WW4 | expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells |
| 166711 | pcDNA3HA-AMOT p130∆PPxY1 | expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells |
| 166712 | pcDNA3HA-AMOT p130∆PPxY2 | expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells |
| 166713 | pcDNA3HA-AMOT p130∆PPxY3 | expresses AMOT with inactivating mutation in third PPxY motif in mammalian cells |
| 166714 | pcDNA3HA-AMOT p130∆PPxY1(L106P) | expresses AMOT(L106P) in mammalian cells |
| 166715 | pcDNA3HA-AMOT p130∆PPxY1(A112K) | expresses AMOT(A112K) in mammalian cells |
| 171130 | pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K) | expresses AMOT(E104K/E105K) in mammalian cells |
| 171137 | pCI-Flag-NEDD4L∆WW1,2,4 | expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells |