Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170808 | pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR | PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance |
| 170809 | pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR | Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen |
| 170810 | pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR | Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I |
| 170811 | pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR | Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen |
| 170812 | pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR | Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I |
| 170813 | pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR | Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I |
| 170814 | pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR | Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen |
| 170815 | pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR | Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I |
| 170816 | pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR | Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I |
| 170817 | pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR | Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I |