Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages.

Johnson MC, Hille LT, Kleinstiver BP, Meeske AJ, Bondy-Denomy J
Mol Cell. 2022 Jun 2;82(11):2161-2166.e3. doi: 10.1016/j.molcel.2022.05.002. Epub 2022 May 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
171128	pCMV-T7-null (BPK1077)	Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.
171129	pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)	LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)