Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida.
Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, Carothers JM, Zalatan JG
Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 10.1016/j.ymben.2021.04.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171138 | pPPC001 | For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method |
| 171139 | pPPC002 | For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method |
| 171140 | pPPC005 | For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method |
| 171141 | pPPC008.106 | Expression of J106 scRNA on pBBR1-GmR plasmid |
| 171142 | pPPC009.106 | Expression of J106 scRNA on pBBR1-KmR plasmid |
| 171143 | pPPC011 | Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid |
| 171144 | pPPC016.109 | Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid |
| 171145 | pPPC017.109 | Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid |
| 171146 | pPPC018.AAV | Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid |
| 171147 | pPPC019.AAV | Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid |
| 171148 | pPPC020.306 | Expression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmid |
| 171149 | pPPC027.306 | Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid |
| 171151 | pPPC030.306 | Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid |
| 171152 | pPPC031 | pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette |
| 171153 | pPPC034 | pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette |
| 171155 | pCK255 | Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid |
| 171175 | pPPC010.AAV | Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid |