Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains.
Santana DJ, O'Meara TR
Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171103 | pTO135_pENO1-CaCas9-tCyc1 | Expresses Cas9 in Candida auris |
| 171104 | pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2 | Expresses sgRNA (ENO1 gRNA) in Candida auris. |
| 171105 | pTO128_pPZP-NATca | A. tumefaciens TI plasmid encoding NATca in T-DNA |
| 177277 | pTO149_RFP_CauNEO | Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency. |