Elongin functions as a loading factor for Mediator at ATF6alpha-regulated ER stress response genes.
He Y, Sato S, Tomomori-Sato C, Chen S, Goode ZH, Conaway JW, Conaway RC
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 10.1073/pnas.2108751118.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171073 | pET-28a (+)-hTBP | Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP |
| 171074 | pET41SIII-ATF6(1-373) | Expresses of ATF6α(1-373) in bacteria |
| 171075 | pET-28a (+)-Gal4-vp16 | Expresses Gal4-vp16 fusion protein in bacteria |
| 171076 | pET-28a (+)-Gal4-p53 | Expresses Gal4-p53 fusion protein in bacteria |
| 171077 | pGal4x5-E4T-G210 | The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission |
| 171081 | pQ-NFY-A-GB-HC | Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC |
| 171082 | pETDuet-TFIIF | Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission |
| 171083 | pETDuet-TFIIE | Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission |
| 171084 | pET-28a (+)-hTFIIB | Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB |
| 171085 | pQ-Elo-HB-C-A(1-772) | Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB |
| 171086 | pQ-Elo-HB-C-A(1-630) | Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB |
| 171087 | pQ-Elo-HB-C-A(400-772) | Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB |