Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.
Chen K, Yan R, Xiang L, Xu K
Light Sci Appl. 2021 May 8;10(1):97. doi: 10.1038/s41377-021-00536-3.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
171058 | pmRuby2-CrowdingLinker-Clover | Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness. |
171059 | pmRuby2-IRES-Clover | Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET. |