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Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.

Chen K, Yan R, Xiang L, Xu K
Light Sci Appl. 2021 May 8;10(1):97. doi: 10.1038/s41377-021-00536-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171058pmRuby2-CrowdingLinker-CloverExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.
171059pmRuby2-IRES-CloverExpresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Antibodies from Article