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Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay.

Teo SLY, Rennick JJ, Yuen D, Al-Wassiti H, Johnston APR, Pouton CW
Nat Commun. 2021 Jun 17;12(1):3721. doi: 10.1038/s41467-021-23997-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170989bdSUMO-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86
170990bdSUMO-R9-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86
170991bdSUMO-TAT-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86
170992bdSUMO-ZF5.3-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86
170993bdSUMO-E5TAT-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86
171014bdSUMO-5.3-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86
171015bdSUMO-pHD118-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86
171016bdSUMO-pHlip-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86
171017bdSUMO-HA2-GFP-HiBitFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86
171019pCDH-EF1-Large BiT - SNAP-tag - Beta-actinLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Antibodies from Article