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A Ratiometric Dual Color Luciferase Reporter for Fast Characterization of Transcriptional Regulatory Elements in Plants.

Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170873pUPD2+E-LucPhytobrick - E-Luc Luciferase CDS
170874pUPD+Red-FPhytobrick - Red-F Luciferase CDS
170875pUPD+pNOSPhytobrick - pNOS
170876pUPD2+p35SoTMVPhytobrick - p35SoTMV
170877pUPD2+pAtUbq10Phytobrick - pAtUBQ10
170878pUPD2+pCmYLCV911Phytobrick - pCmYLCV911
170879pUPD2+pG10-90Phytobrick - pG10-90
170880pUPD2+pEstrIndPhytobrick - Estradiol Inducible Promoter
170881pUPD2+pNRPPhytobrick - pNRP
170882pUPD2+AtAct2tPhytobrick - AtACT2t
170883pUPD2+NtEUtPhytobrick - NtEUt
170884pUPD+AtHSP18.2tPhytobrick - AtHSP18.2t
170885pUPD2+NOStPhytobrick - NOSt
170886pUPD+35StPhytobrick - 35St
170887pDGB1a2+pNOS_Red-F_NOStGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientation
170888pDGB1a2R+pNOS_Red-F_NOStGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientation
170889pGREAT1GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt
170890pGREAT2GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt
170891pGREAT3GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NOSt+pNOS_Red-F_NOSt
170892pGREAT4GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt
170893pGREAT5GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NOSt+pNOS_Red-F_NOSt
170894pGREAT6GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NtEUt+pNOS_Red-F_NOSt
170895pGREAT7GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NtEUt+pNOS_Red-F_NOSt
170896pGREAT8GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NtEUt+pNOS_Red-F_NOSt
170897pGREAT9GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NtEUt+pNOS_Red-F_NOSt
170898pGREAT10GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOSt
170899pGREAT11GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtACT2t+pNOS_Red-F_NOSt
170900pGREAT12GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOSt
170901pGREAT13GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOSt
170902pGREAT14GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOSt
170903pGREAT15GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtACT2t+pNOS_Red-F_NOSt
170904pGREAT16GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt
170905pGREAT17GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt
170906pGREAT18GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt
170907pGREAT19GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt
170908pGREAT20GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt
170909pGREAT21GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_35St+pNOS_Red-F_NOSt
170910pGREAT22GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_35St+pNOS_Red-F_NOSt
170911pGREAT23GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_35St+pNOS_Red-F_NOSt
170912pGREAT24GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_35St+pNOS_Red-F_NOSt
170913pGREAT25GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_35St+pNOS_Red-F_NOSt
170914pGREAT26GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt
170915pGREAT27GoldenBraid Double Transcriptional Unit - pAtNR1_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Antibodies from Article