Alternative splicing is a developmental switch for hTERT expression.
Penev A, Bazley A, Shen M, Boeke JD, Savage SA, Sfeir A
Mol Cell. 2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 10.1016/j.molcel.2021.03.033.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171039 | pRF TpC | Empty vector for Firefly-Renilla luciferase assay for hTERT promoter activity |
| 171040 | pRF TpC RE1 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE1 |
| 171041 | pRF TpC RE2 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE2 |
| 171042 | pRF TpC RE3 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE3 |
| 171043 | pRF TpC RE4 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE4 |
| 171044 | pRF TpC RE5 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE5 |
| 171045 | pRF TpC RE6 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE6 |
| 171046 | pRF TpC RE7 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE7 |
| 171047 | pRF TpC RE8 | Firefly-Renilla luciferase assay for hTERT promoter activity of RE8 |
| 171048 | pTopo pLox hPGK-Puro pA pLox | Empty donor template for CRISPR/Cas9 targeting of TERT RE's |
| 171049 | pTopo RE2 Donor pLox hPGK-Puro pA pLox | Donor template for CRISPR/Cas9 targeting of TERT RE2 |
| 171050 | pTopo RE3 Donor pLox hPGK-Puro pA pLox | Donor template for CRISPR/Cas9 targeting of TERT RE3 |
| 171051 | pLenti MS2-p65-HSF1 sfGFP | Expression of Vp64-dCas9 activation system |
| 171052 | pLenti Vp64-dCas9 T2A mTagBFP2 | Expression of Vp64-dCas9 activation system |
| 171053 | pLenti sgRNA(MS2) mCherry | Expression of Vp64-dCas9 activation system |
| 171054 | pLenti pTERT sgRNA1(MS2) mCherry | Targeting of Vp64-dCas9 activation system to hTERT promoter |
| 171055 | pLenti pTERT sgRNA2(MS2) mCherry | Targeting of Vp64-dCas9 activation system to hTERT promoter |
| 171056 | pcDNA5 FRT-Hygro hTERT dEx2-nanoLuc | hTERT dEx2-expressing splicing minigene |
| 171057 | pcDNA5 FRT-Hygro hTERT FL-Firefly | hTERT Full-length-expressing splicing minigene |
| 171070 | pRF TpC hTERT intron-1 | Firefly-Renilla luciferase assay for hTERT promoter activity of hTERT intron-1 |