CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria.
Trujillo Rodriguez L, Ellington AJ, Reisch CR, Chevrette MG
ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
170981 | pBLlSh2 | Expression of ShCAST under control of lacI Plac promoter/repressor pair. |
170982 | pUCSh2-mRFP | Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning. |
170983 | pUCSh3-mRFP | Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning. |
170984 | pUCSh2-2300 | Encodes sgRNA target 2300 and Kanamycin resistance transposon. |
170985 | pUCSh3-2300 | Encodes sgRNA target 2300 and Tetracycline resistance transposon. |
170986 | pUCSh8-2300 | Encodes sgRNA target 2300 and Hygromycin resistance transposon. |
170987 | pUCSh5vio-2300 | Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon. |
171788 | pUCSh5-2300 | Encodes sgRNA target 2300 and Gentamicin resistance transposon. |
180076 | pRO16Sh5 | Expression of ShCAST under control of Plac with pRO1600 origin. |
180604 | pRO16Sh2 | Expression of ShCAST under control of Plac with pRO1600 origin. |