Rapid generation of potent antibodies by autonomous hypermutation in yeast.

Wellner A, McMahon C, Gilman MSA, Clements JR, Clark S, Nguyen KM, Ho MH, Hu VJ, Shin JE, Feldman J, Hauser BM, Caradonna TM, Wingler LM, Schmidt AG, Marks DS, Abraham J, Kruse AC, Liu CC
Nat Chem Biol. 2021 Jun 24. pii: 10.1038/s41589-021-00832-4. doi: 10.1038/s41589-021-00832-4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170791	pAW240	P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution
172196	pAW212	P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites