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Novel human cell expression method reveals the role and prevalence of posttranslational modification in nonmuscle tropomyosins.

Carman PJ, Barrie KR, Dominguez R
J Biol Chem. 2021 Oct;297(4):101154. doi: 10.1016/j.jbc.2021.101154. Epub 2021 Sep 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170756pJCX4Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
170757pJC8Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
177872pJC7Co-express in mammalian cells two target proteins, using an internal ribosome entry site and two multi-cloning sites.

Antibodies from Article