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Generation of locus-specific degradable tag knock-ins in mouse and human cell lines.

Damhofer H, Radzisheuskaya A, Helin K
STAR Protoc. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575. eCollection 2021 Jun 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170754pUC19-eGFP-P2A-dTAG-Ing3Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus
170755pUC19_dTAG-KANSL3-targetingDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Antibodies from Article