Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein.
Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, Nguyen LT
Elife. 2020 Sep 16;9. pii: 60155. doi: 10.7554/eLife.60155. PubMed Article
Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, Nguyen LT
Elife. 2020 Sep 16;9. pii: 60155. doi: 10.7554/eLife.60155. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170623 | pLIC-lyn11-myc-LOV2GIVe | For the mammalian expression of mLOV2GIVe |
| 170624 | pLIC-lyn11-myc-LOV2GIVe (L) | For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E |
| 170625 | pLIC-lyn11-myc-LOV2GIVe (D) | For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S |
| 170626 | pLIC-lyn11-myc-LOV2GIVe (FA) | For the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A |
| 170627 | pLIC-lyn11-myc-LOV2GIVe (L) (FA) | For the mammalian expression of mLOV2GIVe lit-mimicking mutation I539E and the GEF-deficient mutation F1685A |