SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.
Yan R, Chen K, Wang B, Xu K
Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170570 | pAPV-DsRed2-ER-5 | Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage. |
| 170571 | pEPV-DsRed2-ER-5 | Expresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage. |
| 170620 | pAPV-FLAG-DsRed2-ER-5 | Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage. |
| 170621 | pEPV-FLAG-DsRed2-ER-5 | Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage. |
| 170622 | pAPV-DsRed2-FLAG-ER-5 | Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL. |
| 170675 | pStr-KDEL_APV-SBP-DsRed2-ER-5 | Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH. |
| 170676 | pStr-KDEL_EPV-SBP-DsRed2-ER-5 | Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH. |
| 170677 | pStr-KDEL_TPV-CXCL9-mCherry-SBP | Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH. |
| 170678 | pStr-KDEL_EPV-CXCL9-mCherry-SBP | Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH. |
| 170679 | pStr-KDEL_APV-SBP-Dendra2-ER-5 | Expresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH. |
| 170680 | pFLAG-SURF4 | Expresses FLAG-tagged SURF4 in mammalian cells. |
| 170681 | pAPV-mEmerald-DsRed2-ER-5 | Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL. |
| 170715 | pAPV-DsRed2-mEmerald-ER-5 | Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV. |
| 170717 | pmEmerald-ERGIC-53 | Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells. |