Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila.
Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C
Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 10.1073/pnas.2014255118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170512 | pAC-U63-gRNA2.1 | gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold. |
| 170513 | pAC-U63-QtgRNA2.1-BR | gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues. |
| 170514 | pAC-U63-QtgRNA2.1-8R | gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues. |
| 170515 | pAC-CR7T-gRNA2.1-nlsBFP | gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR. |
| 170516 | pGC(2.1)-U6.3 | PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP |
| 170517 | pTR(EF)-tRNA(Q) | PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80 |
| 194768 | pHACK(Gal4)-DONR(T2A-Cas9) | To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene. |