Computational design of chemogenetic and optogenetic split proteins.
Dagliyan O, Krokhotin A, Ozkan-Dagliyan I, Deiters A, Der CJ, Hahn KM, Dokholyan NV
Nat Commun. 2018 Oct 2;9(1):4042. doi: 10.1038/s41467-018-06531-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170484 | SPELL-Lyn Component 1 | SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279. |
| 170485 | SPELL-Lyn Component 2 | SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279. |
| 170486 | SPELL-GDI Component 1 | SPELL GDI1 N-lobe fused to iFKBP. Split site is 66. |
| 170487 | SPELL-GDI Component 2 | SPELL GDI1 C-lobe fused to FRB. Split site is 66 |
| 170488 | SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1) | SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1 |
| 170489 | SPELL-TEV Component 1 | TEV (N-lobe) fused with iFKBP |
| 170490 | SPELL-TEV Component 2 | TEV (C-lobe) fused with FRB |
| 170491 | SPELL-ITSN1(DH-PH) Component 1 | SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain. |
| 170492 | SPELL-ITSN1(DH-PH) Component 2 | SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain. |