Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model.
Rogers TF, Zhao F, Huang D, Beutler N, Burns A, He WT, Limbo O, Smith C, Song G, Woehl J, Yang L, Abbott RK, Callaghan S, Garcia E, Hurtado J, Parren M, Peng L, Ramirez S, Ricketts J, Ricciardi MJ, Rawlings SA, Wu NC, Yuan M, Smith DM, Nemazee D, Teijaro JR, Voss JE, Wilson IA, Andrabi R, Briney B, Landais E, Sok D, Jardine JG, Burton DR
Science. 2020 Aug 21;369(6506):956-963. doi: 10.1126/science.abc7520. Epub 2020 Jun 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170442 | pcDNA3.3_CoV2_D18 | Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production. |
| 170447 | pcDNA3.3_CoV1_D28 | Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production. |
| 170448 | pCDNA3.3_MERS_D12 | Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production. |
| 170575 | pCMV-FLuc | Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay. |
| 170674 | pBOBI-FLuc | Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay. |