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Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer.

van der Wal J, Habets R, Várnai P, Balla T, Jalink K
J Biol Chem. 2001 May 4. 276(18):15337-44. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170354PIP2-YFP(ST) sensorYFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.
170355PIP2-CFP(ST) sensorCFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.
170356PIP2-GFP(ST) sensorGFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Antibodies from Article