A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range.
Klarenbeek JB, Goedhart J, Hink MA, Gadella TW, Jalink K
PLoS One. 2011 Apr 29;6(4):e19170. doi: 10.1371/journal.pone.0019170.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170338 | Epac-S-H74 | mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |