Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator.
Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G, Zaccolo M, Moolenaar WH, Bos JL, Jalink K
EMBO Rep. 2004 Dec . 5(12):1176-80.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170335 | Epac-S-H21 | CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170336 | Epac-S-H27 | CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170337 | Epac-S-H30 | CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |