Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch.
Dine E, Reed EH, Toettcher JE
Cell Rep. 2021 Jun 22;35(12):109280. doi: 10.1016/j.celrep.2021.109280.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171032 | pHR LAT-FFF/Zap70 iLID-Drop | Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations. |
| 171033 | pHR Src deltaSH2/3-TagBFP | TagBFP-tagged Src variant lacking SH2 and SH3 domains. |
| 171034 | pHR Lck-TagBFP | TagBFP-tagged Lck kinase |
| 171035 | pHR Fyn-TagBFP | TagBFP-tagged Fyn kinase |
| 171036 | pHR Src-TagBFP | TagBFP-tagged Src kinase |
| 171038 | pHR LAT/Zap70 iLID-Drop | Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled. |