Single-component near-infrared optogenetic systems for gene transcription regulation.
Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170268 | pLEVI(408)-ColE-iLight-msfGFP | Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria. |
| 170270 | pBAD/His-D-iLight | Expression of iLight with 6xHis N-terminal tag in bacteria. |
| 170271 | pG12-SEAP | Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid. |
| 170272 | pG12-Rluc8 | Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid. |
| 170273 | pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2 | NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells. |
| 170274 | pAAV-CaMKII-GAL4-iLight-VP16 | NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector. |
| 170275 | pAAV-G12-CheRiff-T2A-mCherry | CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid. |