Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas.
Parekh U, McDonald D, Dailamy A, Wu Y, Cordes T, Zhang K, Tipps A, Metallo C, Mali P
iScience. 2021 Sep 20;24(10):103149. doi: 10.1016/j.isci.2021.103149. eCollection 2021 Oct 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170217 | LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170218 | LV_EF1a_CCNB1-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170220 | LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170221 | LV_EF1a_CDK1-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170222 | LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170223 | LV_EF1a_CDK4-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170224 | LV_EF1a_CDK6-P2A-Hygro_Barcode | Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170225 | LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170226 | LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170228 | LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170229 | LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170236 | LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170237 | LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170238 | LV_EF1a_MAPK9-P2A-Hygro_Barcode | Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170239 | LV_EF1a_MAPK14-P2A-Hygro_Barcode | Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170240 | LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170248 | LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170249 | LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170254 | LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode | Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |
| 170255 | LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode | Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq |