Shulin packages axonemal outer dynein arms for ciliary targeting.
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170311 | pBluescript-IC3-FLAG-Tev-ZZ | Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags. |
| 170312 | pBluescript-Q22MS1-KO | Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection |
| 170313 | pBluescript-Q22YU3-KO | Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection |
| 170314 | pACEBac1-Q22MS1-2xStrep | Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II |
| 170315 | pACEBac1-Q22YU3-2xStrep | Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II |
| 170316 | pACEBac1-Q22YU3 del C3-2xStrep | Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus. |