Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits.
Chen J, Poskanzer KE, Freeman MR, Monk KR
Nat Neurosci. 2020 Oct;23(10):1297-1306. doi: 10.1038/s41593-020-0703-x. Epub 2020 Sep 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170205 | p5E-slc1a3b | Gateway 5’ entry vector with the slc1a3b promoter |
| 170206 | pME-mCD8mCherry | Gateway middle entry vector that encodes mCD8mCherry |
| 170207 | slc1a3b:mCD8mCherry | Drives mCD8mCherry expression with the slc1a3b promoter in astrocytes |
| 170208 | slc1a3b:myrGFP-P2A-H2AmCherry | Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus |
| 170209 | slc1a3b:myrGCaMP6s-P2A-H2AmCherry | Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus |