Bacterial Retrons Enable Precise Gene Editing in Human Cells.
Zhao B, Chen SA, Lee J, Fraser HB
CRISPR J. 2022 Jan 24. doi: 10.1089/crispr.2021.0065.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170182 | pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry | Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide |
| 170183 | pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry | Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide |
| 170184 | pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry | Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII. |
| 170185 | pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry | Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII. |