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Engineering light-controllable CAR T cells for cancer immunotherapy.

Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang X, Chien S, Wang Y
Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. eCollection 2020 Feb. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171757pHR-PGK-LexA-WW-CIB1-biLINuSLentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuS
171758pHR-PGK-WP1-CRY2PHR-VP64Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64
171759pHR-LexAOx4-Pmin-CreLentiviral vector for inducible Cre expression with 4x LexA operator (LexAO)
192604pDN-LexAOx4-mNeonGreenVector for inducible mNeonGreen expression with 4x LexA operator (LexAO)
192605pCBI-LexA-CIB1-biLINuSPlasmid for constitutive expression of LexA-CIB1-biLINuS.
192606pCBI-CRY2PHR-VPRPlasmid for constitutive expression of CRY2PHR-VPR.
192607pHR-PGK-LexA-CIB1-biLINuSLentiviral vector for constitutive expression of LexA-CIB1-biLINuS
192608pHR-PGK-CRY2PHR-VPRLentiviral vector for constitutive expression of CRY2PHR-VPR.
192609pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFPLentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.
192610pHR-PGK-eGFP-P2A-CRY2-VPRLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.
192633pSIN-LexAOx4-Pmin-mCherryLentiviral vector for inducible mCherry expression.
192634pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherryLentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

Antibodies from Article