High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.

Sridharan S, Gajowa MA, Ogando MB, Jagadisan UK, Abdeladim L, Sadahiro M, Bounds HA, Hendricks WD, Turney TS, Tayler I, Gopakumar K, Oldenburg IA, Brohawn SG, Adesnik H
Neuron. 2022 Jan 27. pii: S0896-6273(22)00008-3. doi: 10.1016/j.neuron.2022.01.008. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170160	pCAG-ChroME2f-mRuby2-ST	Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
170161	pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3	Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
170163	pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3	Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
170175	pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3	Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
170177	pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3	Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
171150	pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3	Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
171154	pCAG-mRuby3	Encodes mRuby3 fluorophore
171156	pCAG-GCaMP6s	Encodes GCaMP6s
171158	pCAG-ChroMD-mRuby2-ST	Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
227777	pCAG-ChRmine-mRuby2-ST	Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation