A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes.
Wimmer F, Englert F, Beisel CL
Methods Mol Biol. 2022;2433:391-411. doi: 10.1007/978-1-0716-1998-8_24.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170088 | pEc-crRNA1 | encodes E. coli type I-E single spacer array |
| 170089 | pEc-crRNA2 | encodes E. coli type I-E single spacer array |
| 170090 | pEcCas5 | expresses E. coli type I-E Cas5 |
| 170091 | pEcCas6 | expresses E. coli type I-E Cas6 |
| 170092 | pEcCas7 | expresses E. coli type I-E Cas7 |
| 170093 | pEcCas8 | expresses E. coli type I-E Cas8 |
| 170094 | pEcCse2 | expresses E. coli type I-E Cse2 |
| 170095 | pGFP-ATAAC | deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter |
| 170096 | pGFP-CAAAG | deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter |
| 170097 | pGFP-CAATG | deGFP reporter plasmid with CAATG PAM upstream of p70a promoter |
| 170098 | pGFP-GTAAT | deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter |
| 170099 | pGFP-GTATT | deGFP reporter plasmid with GTATT PAM upstream of p70a promoter |
| 170100 | pGFP-PacI | deGFP reporter plasmid with unique PacI restriction enzyme recognition site |
| 170101 | pT7RNAP | expresses T7 RNAP |