Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens.
Morris JA, Caragine C, Daniloski Z, Domingo J, Barry T, Lu L, Davis K, Ziosi M, Glinos DA, Hao S, Mimitou EP, Smibert P, Roeder K, Katsevich E, Lappalainen T, Sanjana NE
Science. 2023 May 19;380(6646):eadh7699. doi: 10.1126/science.adh7699. Epub 2023 May 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170067 | lentiCRISPRi(v1)-Blast | Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance |
| 170068 | lentiCRISPRi(v2)-Blast | Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance |
| 170069 | lentiGuideFE-Puro | Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter |
| 199303 | lentiBE3-SpRY-Blast | Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance |